Effects of a novel combination of two mutated acetolactate synthase (ALS) isozymes on resistance to ALS-inhibiting herbicides in flixweed (<i>Descurainia sophia</i>)

Abstract Flixweed [ Descurainia sophia (L.) Webb ex Prantl] is a notorious broadleaf weed that widely distributed in winter wheat–growing areas of China and has evolved resistance to tribenuron-methyl mainly due target-site (TSR) mutations acetolactate synthase (ALS). In the current research, two ALS genes were identified tribenuron-methyl–susceptible (TS) or tribenuron-methyl–resistant (TR) D. . Resistance Asp-376-Glu Pro-197-Ala on ALS1 ALS2 isozymes TR , respectively. The 10,836.3-fold displayed cross-resistance multiple ALS-inhibiting herbicides with different chemical structures. Dose response experiments activity assay indicated mutated contributed differentially tribenuron-methyl, flucetosulfuron, pyribenzoxim. addition, relative expression level gene was 2.2- 1.6-fold higher than at 1 7 d after treatment, contrast, TS similar. This first research explored roles herbicides, which might provide new perspective for management.